Thank you for your reply, Karen.

Yes..Ayla is spayed. We are most definitely going to take them both to training classes. It was interesting how I could only find info on the older dog showing aggression to the new puppy, but not to the owners. I am thinking that Beau and Ayla may have already established that he is alpha.(as he is a rottweiller mix) and will def grow bigger than she. So, perhaps Ayla is trying to re-establish herself in the pack order. AND in that lil defiant stage!

This was indeed helpful!! Thanks again, Karin!