We just moved with our kitten. We took her food & water up about 12 hours before we left. I highly recommend that you do this. Josie would have thrown up if she would have had anything in her belly. She was making the motions but nothing was coming out. Maybe you could feed them the night before, and take it up before you go to work. And for just a two hour ride, you don't need to worry about feeding them & watering them during the ride. Most cats won't eat or drink for a couple of hours after they've arrived anyway. Too much excitement.