Yes, 9 out of 10 times I can tell them apart by their voices. But sometimes I think they do a ventriloquist routine to get some else into trouble. I can tell who they are by petting down their back and up their tail. The only trouble I have, in the dark, is that Willow and Ashley have the same facial markings, so even though one is tabby and white and one is calico, head on they could be the same. So I put a color on Ashley to help tell them apart. I used to get Y.Lee and Amber mixed up if they were laying down because they were just one big blob of orange. So now I go for the one of every color route.