Kim - whatever it is will be manageable. And if some treatment also settles his 'nerves' a bit - if he is feeling unwell, that would affect his mood - he will adjust better.
Obviously there was a time when he and Pouncer got along for a bit...one cat at a time. Your idea to take him to all the rooms on his own without the other cats sounds like a good one.
Prayers for you and beautiful Tuscan!
PS How is Pouncer?
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