The puppies eyes will open up by day 16. You will start to see slits and then by the third week the eyes will be wide open. The ears will open following the eyes. Eyes come first. By three weeks both eyes and ears are open.
The most important thing the new parents of the puppies need to do is socialize the little ones properly before they go to their new homes. This is of course understanding that their eating and cleanliness are taken care of.
Introduce the little ones to as many stimulating things as possible. Sounds, lights, toys, etc... Don't let other dogs around them yet though since they are not vacinated and can easily come down with many life threatening diseases. Limit the people who come to see them too until they get their first set of shot at 6 weeks. Make sure their environment is kept clean and sanitary. This becomes even more critical once the pups start weening around 4 or 5 weeks. By week 7 they should be weened from mom completely. By week 8 they should be ready to go to their new home.
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